A few olpidium species are recognized as fungal vectors of plant viruses. Olpidium virulentus olpidium brassicae, a unicellular fungus parasitizing. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. Alternatively, you can download the pdf file directly to your computer, from where it can be opened using a pdf reader. Accurate taxonomic knowledge is the foundation for further discussions and studies of pathogenicity and ecological role. Olpidium bornovanus, a unicellular fungus, is an obligate parasite of plants that reproduces with flagellated. Host specificity and nomenclature of olpidium bornovanus. For example, olpidium brassicae transmits bigvein virus and bigvein associated varicosavirus among lettuce. A phylogeny from four proteincoding genes shows that olpidium is the only flagellated genus in a clade of terrestrial nonflagellated fungi.
Specifically, colonization of cantaloupe roots by zoospores of o. The protruding domain of the coat protein of melon. We developed a quantitative detection system for olpidium bornovanus. A multigene phylogeny of olpidium and its implications for early. Melon necrotic spot virus mnsv can cause significant economic losses in melon c. Olpidium bornovanus olpibodocuments eppo global database. Olpidium is a fungal genus in the family olpidiaceae. We do not use these to store personal information about you. Topology tests rejected monophyly of the olpidium species with any other clades of flagellated fungi. Olpidiomycota, rozellomycota and zoopagomycota pdf. A cucumber necrosis virus variant deficient in fungal.
Download fulltext pdf download fulltext pdf first report of olpidium bornovanus and o. Olpidium brassicae is commonly present in roots of field crops, especially in the members of brassicaceae, including canola. Bait melon plants grown in the contaminated soil were colonized by both olpidium species and by m. For example, olpidium brassicae transmits bigvein virus and big vein associated varicosavirus among lettuce. In this thesis, the molecular biology of the interaction between cucumber necrosis tombusvirus cnv and zoospores of its fungal vector, olpidium bornovanus, was examined. Involvement of the cucumber necrosis virus coat protein in. Association of olpidium bornovanus and melon necrotic spot virus with vine decline of melon in guatemala. Pdf first report of olpidium virulentus on lettuce in turkey. The symptoms are chlorotic or necrotic spots in leaves, necrotic streaks in stems and occasionally plant death. Olpidium bornovanus is the first vector reported for csbv and is confirmed as a vector of sqnv. Relationships between the pathogen olpidium virulentus and viruses associated with lettuce bigvein disease.
Relationship between the lettuce bigvein virus and its vector, olpidium brassicae. To our knowledge, no previous studies have reported natural mixed infections of olpidium. The in vitro virusvector relationship is found between the isometric viruses of the tombusviridae and two olpidium. Pdf a multigene phylogeny of olpidium and its implications for. Phyto86n12 1294 american phytopathological society.
Two experiments for watermelon and two experiments with melon were carried out in soils infected with fusarium oxysporum f. Cnv is transmitted by the chytrid fungus olpidium bornovanus. Our laboratory has developed a novel multiplex pcr assay for the rapid and simultaneous detection and differentiation of three olpidium spp. They appear to be directly transmitted through the soil. Taxonomy and pathogenicity of olpidium brassicae and its allied. Olpidium bornovanus and melon necrotic spot virus mnsv in. Melon necrotic spot virus mnsv is a virus that belongs to the genus carmovirus of the family tombusviridae. In nature the virus is transmitted by the fungus olpidium radicale o.
Pdf taxonomy and pathogenicity of olpidium brassicae and its. A stepwise detection system of obligate parasitic fungi in. General information about olpidium bornovanus olpibo. Relationship between the lettuce bigvein virus and its vector. Studies on melon necrotic spot virus disease of cucumber. Studies on melon necrotic spot virus disease of cucumber and on the control of the fungus vector olpidium radicale authors. Thirty soil borne viruses or virus like agents are transmitted by five species of fungal vectors i. Virus transmission by hostspecific strains of olpidium bornovanus. The vector also formed the basis for the most sensitive assays of seed borne virus, enzymelinked immunosorbent assay elisa.
Documents about olpidium bornovanus olpibo this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Host specificity and nomenclature of olpidium bornovanus olpidium radicale and comparisons to olpidium brassicae. Effect of soil matric water potentials on germination of. One of the specific amplicons of the expected size 579 nt. Presentation mode open print download current view. Results document, for the first time, the role of soil moisture on a unique, tripartite, hostspecific rhizosphere interaction i. This observation suggested that germination is mediated by one or more heatsensitive members of the soil microflora.
Olpidium bornovanus olpibooverview eppo global database. Some species may be transmitted through the seed at a very. A multigene phylogeny of olpidium and its implications for early fungal evolution satoshi sekimoto1,3,dann rochon2, jennifer e long1,4, jaclyn m dee1 and mary l berbee1. Although bacteria or actinomycetes were heretofore suspected as the germinationinducing microbes, our data demonstrate that olpidium bornovanus. Ishs xxviii international horticultural congress on science and horticulture for people ihc2010. To our knowledge, no previous studies have reported natural mixed infections of olpidium spp.
First report of olpidium virulentus on lettuce in turkey springerlink. Thus, the combination olpidium bornovanus sahtiyanci karling is accepted. Molecular characterization of the interaction between. From a common ancestor with animals, the earliest fungi inherited flagellated. The melon necrotic spot virus is the cause of a serious disorder in greenhouse melon crops. Multiplex pcr assay for the simultaneous detection and. Cucumber necrosis cnv and the cherry strain of tomato bushy stunt tbsvch are tombusviruses which differ in transmissibility by the fungus olpidium bornovanus sahtiyanci karling o. Download fulltext pdf download fulltext pdf a multigene phylogeny of olpidium and its implications for early fungal evolution article pdf available in bmc evolutionary biology 111. Molecular aspects of plant virus transmission by olpidium and. Olpidium bornovanusmediated germination of ascospores of. A multigene phylogeny of olpidium and its implications for. Watermelon production with rootstocks in soils infested. Olpidium brassicae is a common fungal parasite of the epidermal cells of plant roots, especially plants in the family brassicaceae formerly cruciferae. This virus attacks crops of cucumber cucumis sativus l.
Resistance to melon necrotic spot virus in cucumis melo l. In particular, we clarify the differences between o. Finding of lettuce big vein virus in czechoslovakia. It has been observed in several countries of the americas, africa, asia, and europe. A multigene phylogeny of olpidium and its implications for early fungal evolution. Virus transmission by hostspecific strains of olpidium. Department of energy joint genome institute, a doe office of science user facility, is supported by the office of science of the u. Taxonomy and pathogenicity of olpidium brassicae and its. This article is from bmc evolutionary biology, volume 11. Continuing to use this website means you agree to our use of cookies.
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