A multigene phylogeny of olpidium and its implications for early fungal evolution. Olpidium bornovanus, a unicellular fungus, is an obligate parasite of plants that reproduces with flagellated, swimming zoospores. The in vitro virusvector relationship is found between the isometric viruses of the tombusviridae and two olpidium. Olpidium bornovanus olpibooverview eppo global database. Cucumber necrosis cnv and the cherry strain of tomato bushy stunt tbsvch are tombusviruses which differ in transmissibility by the fungus olpidium bornovanus sahtiyanci karling o. Specifically, colonization of cantaloupe roots by zoospores of o. Olpidium bornovanus and melon necrotic spot virus mnsv in. Melon necrotic spot virus mnsv can cause significant economic losses in melon c. Continuing to use this website means you agree to our use of cookies.
Some samples tested in this study were found to be dually infected with o. International symposium on plant protection influence of grafted watermelon plant. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. In nature the virus is transmitted by the fungus olpidium radicale o.
This article is from bmc evolutionary biology, volume 11. Virus transmission by hostspecific strains of olpidium. General information about olpidium bornovanus olpibo. Pdf first report of olpidium virulentus on lettuce in turkey. Watermelon production with rootstocks in soils infested. Download fulltext pdf download fulltext pdf first report of olpidium bornovanus and o. Relationship between the lettuce bigvein virus and its vector. Taxonomy and pathogenicity of olpidium brassicae and its allied.
A multigene phylogeny of olpidium and its implications for. Olpidiomycota, rozellomycota and zoopagomycota pdf. We developed a quantitative detection system for olpidium bornovanus. Aleandri mp, martignoni d, reda r, alfarofernandez a, font mi, armengol j chilosi g 2017 involvement of olpidium bornovanus and o. Thirty soil borne viruses or virus like agents are transmitted by five species of fungal vectors i.
Relationships between the pathogen olpidium virulentus and viruses associated with lettuce bigvein disease. Olpidium bornovanus is the first vector reported for csbv and is confirmed as a vector of sqnv. A multigene phylogeny of olpidium and its implications for early fungal evolution satoshi sekimoto1,3,dann rochon2, jennifer e long1,4, jaclyn m dee1 and mary l berbee1. Alternatively, you can download the pdf file directly to your computer, from where it can be opened using a pdf reader. Melon necrotic spot virus mnsv is a virus that belongs to the genus carmovirus of the family tombusviridae. Relationship between the lettuce bigvein virus and its vector, olpidium brassicae. Studies on melon necrotic spot virus disease of cucumber and on the control of the fungus vector olpidium radicale authors. Accurate taxonomic knowledge is the foundation for further discussions and studies of pathogenicity and ecological role.
A cucumber necrosis virus variant deficient in fungal. Olpidium bornovanusmediated germination of ascospores of. Bait melon plants grown in the contaminated soil were colonized by both olpidium species and by m. Download fulltext pdf download fulltext pdf a multigene phylogeny of olpidium and its implications for early fungal evolution article pdf available in bmc evolutionary biology 111. Olpidium is a fungal genus in the family olpidiaceae. For example, olpidium brassicae transmits bigvein virus and bigvein associated varicosavirus among lettuce. One of the specific amplicons of the expected size 579 nt. In this thesis, the molecular biology of the interaction between cucumber necrosis tombusvirus cnv and zoospores of its fungal vector, olpidium bornovanus, was examined. Olpidium virulentus olpidium brassicae, a unicellular fungus parasitizing. Taxonomy and pathogenicity of olpidium brassicae and its. A multigene phylogeny of olpidium and its implications for early.
Molecular aspects of plant virus transmission by olpidium and. Some species may be transmitted through the seed at a very. Our laboratory has developed a novel multiplex pcr assay for the rapid and simultaneous detection and differentiation of three olpidium spp. Olpidium bornovanus, a unicellular fungus, is an obligate parasite of plants that reproduces with flagellated. This observation suggested that germination is mediated by one or more heatsensitive members of the soil microflora. Pdf taxonomy and pathogenicity of olpidium brassicae and its. Although bacteria or actinomycetes were heretofore suspected as the germinationinducing microbes, our data demonstrate that olpidium bornovanus.
Ishs xxviii international horticultural congress on science and horticulture for people ihc2010. Cnv is transmitted by the chytrid fungus olpidium bornovanus. For the control of viral diseases caused by olpidium species, it is important to quantitatively detect the presence of such fungi in field soil. Olpidium brassicae is a common fungal parasite of the epidermal cells of plant roots, especially plants in the family brassicaceae formerly cruciferae. Host specificity and nomenclature of olpidium bornovanus. Two experiments for watermelon and two experiments with melon were carried out in soils infected with fusarium oxysporum f. Results document, for the first time, the role of soil moisture on a unique, tripartite, hostspecific rhizosphere interaction i. The melon necrotic spot virus is the cause of a serious disorder in greenhouse melon crops. Olpidium brassicae is commonly present in roots of field crops, especially in the members of brassicaceae, including canola. It has been observed in several countries of the americas, africa, asia, and europe. Olpidium bornovanus olpibodocuments eppo global database. Involvement of the cucumber necrosis virus coat protein in.
For example, olpidium brassicae transmits bigvein virus and big vein associated varicosavirus among lettuce. Documents about olpidium bornovanus olpibo this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Topology tests rejected monophyly of the olpidium species with any other clades of flagellated fungi. In particular, we clarify the differences between o. They appear to be directly transmitted through the soil. First report of olpidium virulentus on lettuce in turkey springerlink. A stepwise detection system of obligate parasitic fungi in. Studies on melon necrotic spot virus disease of cucumber. To our knowledge, no previous studies have reported natural mixed infections of olpidium. To our knowledge, no previous studies have reported natural mixed infections of olpidium spp. The vector also formed the basis for the most sensitive assays of seed borne virus, enzymelinked immunosorbent assay elisa. Finding of lettuce big vein virus in czechoslovakia. The symptoms are chlorotic or necrotic spots in leaves, necrotic streaks in stems and occasionally plant death. Virus transmission by hostspecific strains of olpidium bornovanus.
From a common ancestor with animals, the earliest fungi inherited flagellated. We do not use these to store personal information about you. Presentation mode open print download current view. Analysis of chimeric viruses constructed by switching the coat protein cp gene between the two strains unveiled the involvement of the cp in the attachment of mnsv to zoospores of a compatible isolate of o. Multiplex pcr assay for the simultaneous detection and. A few olpidium species are recognized as fungal vectors of plant viruses. Host specificity and nomenclature of olpidium bornovanus olpidium radicale and comparisons to olpidium brassicae. Phyto86n12 1294 american phytopathological society. This virus attacks crops of cucumber cucumis sativus l. Thus, the combination olpidium bornovanus sahtiyanci karling is accepted. Department of energy joint genome institute, a doe office of science user facility, is supported by the office of science of the u. Molecular characterization of the interaction between. Association of olpidium bornovanus and melon necrotic spot virus with vine decline of melon in guatemala. Pdf a multigene phylogeny of olpidium and its implications for.
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